FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n02_zt14ss44.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n02_zt14ss44.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1663612
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23390.14059768744154286No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGATAT450.004623566330.66627945
CGATCTG46200.021.9549275
GTCCGTA850.00411981320.2950821
TGTCGCC2402.7284841E-1120.12474410
GTCGCCC2303.45608E-1019.49975411
AATACCG1258.742847E-519.3203375
ATACCGT1108.237874E-418.818516
TTACGGC1301.1812717E-418.57724618
CCGATCT124400.018.165514
CTATACT6650.017.6394044
GATCTGG19250.017.5639446
CGATCTT24050.017.5013455
TCTCGCG1402.0830064E-417.2503018
TTCCGAT131100.017.2113442
TCCGATC132100.017.1327743
CGTAGGG1653.8416936E-516.7280672
CTTCCGA139650.016.4540581
TCGCCCA2955.366019E-1016.37267512
GTACTAG2907.528797E-915.4662511
GTATAAA7900.015.2855371
Produced by FastQC (version 0.11.2)