FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n02_zt14ss43.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n02_zt14ss43.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1027816
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16440.15995080831588535No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGTTG700.001333772824.64278815
GTGCCGA1202.4574783E-622.99993766
CTACGGT750.001992912722.99993534
TGCGGGA1103.2665484E-521.95448313
TAGACGT4750.021.7894134
CGTGCGG800.00289927321.5624411
TAATCCG800.00289927321.5624448
TCGTGCG850.004119957320.29406210
CGATCTT14600.019.6129595
CTAGACG3800.019.0657373
GTCCTAT2002.8749128E-818.9777161
GTATTAA4100.018.5148451
CCTTACG5600.018.4820969
ACGAGTC950.0078340318.15784550
CCGACGC1200.001476661717.24995269
CGACTCG1402.082353E-417.2499559
ATCACCG1452.7221223E-416.65512512
ATCCGCT1250.001940460416.55995450
CGATCTG29900.016.4999545
ATCTCGC3351.0913936E-1116.4775667
Produced by FastQC (version 0.11.2)