FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n02_zt14ss42.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n02_zt14ss42.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1735883
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21520.12397148886186453No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTGCG1451.2654104E-519.0344839
CGATCTG57450.018.135775
CGATCTT23850.017.5031455
CGTAACG1402.0833498E-417.24999853
TATACTG6450.016.5813945
ATACCGT1250.001941149816.566
CCGATCT139050.016.2017254
GTATATA10850.015.5806461
TCGGGTA2001.1426282E-515.52499925
TTCCGAT146250.015.3569232
TCCGATC146700.015.3098173
GTCTTAT6100.015.2704921
TAATCCG1605.760165E-415.0937548
TTATACT6200.015.0241944
CTTCCGA151700.014.9644031
TGGTCGC1400.004131968614.78571310
CGAAGAG36600.014.61065569
GTACATA5200.014.5961541
TCACGAA1901.2822256E-414.52631657
CGGGTAA2404.0056057E-614.37526
Produced by FastQC (version 0.11.2)