FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n02_zt14ss41.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n02_zt14ss41.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8491449
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG111000.13071973935190567No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTT130550.018.3135915
CGATCTG285050.018.0578045
GATCTGG92950.016.5539886
CCGATCT719950.016.0722944
TAGACGT34650.015.8310994
TTCCGAT753250.015.4905552
TCCGATC759250.015.34942153
CTAGACG26100.015.0688983
GATCTTT97450.015.0107076
CTTCCGA794850.014.8802821
TCTGTCG17900.014.6479818
ATCTGCG26850.014.3909987
TAATACT36550.014.2530154
GTATTAG36300.013.9722461
GTGTATA63200.013.6482961
GTATATA83450.013.6440451
CGAATAG151050.013.5441369
GTATAGG26250.013.5382791
TGTCGCC12950.013.32032510
TCTGCGC16450.013.2127078
Produced by FastQC (version 0.11.2)