FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n02_zt14ss40.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n02_zt14ss40.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77577
Sequences flagged as poor quality0
Sequence length75
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG67328.677829769132604No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTTAA208.4400206E-569.0427
CATGCGT150.002347861668.9954936
CTCCCTT150.002347861668.9954911
TGTATCG150.002347861668.995495
CTAAGAA150.002347861668.995492
TCAGACA150.002347861668.995492
GTATCGT208.467054E-568.995496
TATCGTG150.002347861668.995497
TCCGGCT252.5449338E-455.23199524
TAGGTTT200.007335219551.7466164
CAATTTG200.007335219551.74661666
TGTGCTA200.007335219551.7466168
AAACGAA200.007335219551.74661655
GTGCTCT200.007335219551.7466164
TTGACTC200.007335219551.74661653
ATAAGAT200.007335219551.7466163
CTCTGTA200.007335219551.7466162
CACGTTT200.007335219551.74661648
TGTACTC200.007335219551.74661661
GTGTCTC306.2769593E-445.996993
Produced by FastQC (version 0.11.2)