FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n02_zt14ss3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n02_zt14ss3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2676796
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG40960.15301875824679953No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG48850.029.7337555
CGATCTT28300.029.3807495
CTGCGCG953.2056414E-729.0535139
CCGATCT120050.027.6750414
TTCCGAT122500.027.1783752
TCCGATC125000.026.5515083
CTTCCGA138650.024.3609981
CGACGAA851.4442508E-424.35231669
CGTGTAG5050.023.22712769
GATCTGG26150.022.2970436
GTACGCG1403.778514E-722.17924528
ATCTGCG5550.021.7574167
CGATCTA19600.020.595015
TACGTGT12100.020.52839769
CCGACGC1952.158231E-819.46103969
GCGTTCG900.005735360619.167259
TCTGCGC3150.018.6196128
TACGCGA1501.694844E-518.40055829
TCTCGCG1501.694844E-518.4005588
TTGTCGG6200.018.36380422
Produced by FastQC (version 0.11.2)