FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n02_zt14ss39.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n02_zt14ss39.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75151
Sequences flagged as poor quality0
Sequence length75
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG57937.708480259743716No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTAGG150.002347148768.99866533
GTGCTCC150.002347148768.99866516
GTGCGGA208.463417E-568.99866553
AACACGC150.002347148768.99866510
TCACACT252.5435846E-455.2356832
TTTCAAG252.5519723E-455.19893626
CCCGGAA252.5519723E-455.19893614
ACTGATA200.00733300351.7499
GTAGGTT200.00733300351.74941
CCTAATG200.00733300351.74912
CCAGATT200.00733300351.74921
GTTACAC200.00733300351.74933
GCATTCA200.00733300351.74922
ACCTAAT200.00733300351.74911
TACTGAT200.00733300351.7498
CCGAGGG200.00733300351.74917
TGTTACA200.00733300351.74932
TGGATTA200.00733300351.74947
CTCGAAT200.00733300351.74924
TCATACA200.00733300351.74952
Produced by FastQC (version 0.11.2)