FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n02_zt14ss38.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n02_zt14ss38.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2439583
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG34190.14014690215499945No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG78900.021.2947335
GATCTGG30100.019.9435756
CGATCTT41800.018.3230135
CCGATCT209950.017.5170754
TTCCGAT220850.016.6226442
TCCGATC222900.016.5767613
GGACCGT2603.304922E-815.923126
CTTCCGA234000.015.9103321
TCTGTCG4650.015.58068758
ATCTGCG7800.015.480817
ATCTCGC8850.015.203437
ATCTGGC16100.015.000047
CTGTCGC4850.014.9381859
TGTCGCC4400.014.89746210
GATAGCG1400.004132531614.7857557
TACACCG2101.8136037E-514.7857545
CGCTCGG1657.2732545E-414.63700319
AACGATA1657.2766445E-414.63610361
GTATATA14050.014.2437831
ACCGTTC1709.120547E-414.205928
Produced by FastQC (version 0.11.2)