FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n02_zt14ss37.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n02_zt14ss37.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2157011
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG63190.2929516817484936No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG69750.027.4017725
CCGATCT217900.023.289954
GATCTGG28650.023.12016
CGATACG1502.890556E-822.99968169
TTCCGAT226250.022.5381952
TTTCGGA6900.022.5017761
CGATCTT73600.022.4059415
TTCGGAA7350.022.0619432
TCCGATC232850.021.8094483
GATCTTT57200.021.4717316
TCTGCGC6600.020.9088028
CTTGTGT28900.020.41321269
GATCTGC33950.020.2221076
ATCTGCG10200.019.9556087
CTGTCCG1751.4744546E-719.7140149
GATCTCG19400.019.5615866
ATCTTTT61950.019.4355687
CTTCCGA268450.019.2402971
GAGTCGC900.005736469319.16640329
GAGCGTA900.005736469319.1664039
Produced by FastQC (version 0.11.2)