FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n02_zt14ss36.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n02_zt14ss36.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2030620
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30680.1510868601707853No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCCG1004.3364763E-420.6999329
GGTAACG1004.3364763E-420.69993226
GTTCGAT1004.3364763E-420.69993243
TATCCGG900.00573600719.1666055
ACGAACC900.00573600719.16660533
CGATCTG72300.018.9916385
GACGGTT1954.597514E-717.69225168
CGATCTT34950.017.6694725
GGTCGTC1250.001941343716.55994646
GATCTGG23100.016.2791676
CCGATCC5750.016.19994733
CCGATCT189400.016.1570224
AACCGAT1503.526879E-416.09994950
ATCTGCG6500.015.9230257
GTGTAAG7600.015.4360621
TTCCGAT199800.015.3520642
TCCGATC201600.015.3161713
CTAGACG7300.015.1232393
GTATTAA7400.014.9207091
ACGGTTG1851.021954E-414.91887169
Produced by FastQC (version 0.11.2)