FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n02_zt14ss35.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n02_zt14ss35.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2122764
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26560.1251198908592759No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACCG953.0662178E-421.790095
GTATTAG6100.020.3621921
CGCCGTT1551.0050153E-620.03282525
GCGACGA1108.240903E-418.81782766
CGATCTG60600.018.6737965
GGACCGA950.00783530218.1584076
GATCTGG21550.017.4506116
CGATCTT30700.017.4190625
GTATTAA9000.017.25131
GTATCGC1402.083174E-417.2504886
CGGTACG1200.001477521617.24967638
TGGTCGG1200.001477521617.24967610
TTACGTG6650.017.1199869
ACGACGC1653.8446815E-516.7269658
CCGACGC3157.6397555E-1116.42826369
CGTGTAG3651.8189894E-1216.06819269
CCGATCT162350.015.9807424
ATCTGCG5400.015.9726757
GTATATA16100.015.6440371
GTATTAT9050.015.6310141
Produced by FastQC (version 0.11.2)