FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n02_zt14ss34.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n02_zt14ss34.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1705431
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21020.12325330077851288No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGCGC1001.5642538E-524.149978
TACACCG1756.206392E-921.6856865
TACGAGT1154.6043555E-520.99997349
TCTGCGC2352.0008883E-1120.5531658
CGATCTG55100.018.8466195
TTGTCGC1108.239023E-418.8181610
ACACCGT1852.644938E-718.6486246
CGATCTT23800.017.9747685
ATCTGCG4700.016.8829577
CACGTCG1653.8433904E-516.72725128
CCGATCT137500.016.4094334
GTCTTAC5200.015.923991
CTGATCA7800.015.9230589
TCCGGGT1300.002522403815.92305713
GATCTGG18950.015.6569726
TTCCGAT144450.015.5964912
TCCGATC145600.015.5676313
ACTGATC6650.015.563898
GTCTTGC7100.015.5501881
ATCTCGC5550.015.5405227
Produced by FastQC (version 0.11.2)