FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n02_zt14ss33.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n02_zt14ss33.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8741757
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG137320.15708512602214864No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG215200.020.3280625
CGATCTT105850.019.8819055
CCGATCT548950.017.6726444
CGTAACG2156.607479E-817.65095553
TTCCGAT571750.017.0585122
TCCGATC580350.016.8353543
CTTCCGA603050.016.4828111
GATCTGG85750.016.334696
ATCTGCG22750.014.5582377
TAGACGT24650.014.55577854
GTATTAG33900.014.4520681
GTATTAT36250.013.9910611
CGAATAG155000.013.95564569
GTGTATA61400.013.71081
ACTGATC48000.013.4406218
GTATAAG27050.013.0099091
TACACCG6400.012.9374975
GTATATA70250.012.9166761
GATCTTT89500.012.9134046
GGGGTGC50150.012.7268176
Produced by FastQC (version 0.11.2)