FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n02_zt14ss32.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n02_zt14ss32.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50242
Sequences flagged as poor quality0
Sequence length75
%GC59

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG565511.255523267385852No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCCTC150.00234241569.063
CTCTTTT150.00234241569.049
AGTGCCT150.00234241569.051
TCAGACG150.00234241569.019
ATGCGAC150.00234241569.069
GGTCTGA150.00234241569.026
GATTCCT150.00234241569.020
CAGTGCC252.5435365E-455.19999750
GGAGCGA200.007318326751.7500046
ATACTGC200.007318326751.7500048
CCCAATG200.007318326751.75000453
GCCCTTA200.007318326751.75000442
ATCTTAC200.007318326751.75000468
GCAGGTG200.007318326751.75000423
CGAGCCT200.007318326751.75000410
AAGGGTC200.007318326751.75000432
AGAATAC200.007318326751.7500045
CACCCGC200.007318326751.75000424
GGTAGGG200.007318326751.75000415
AGAGTGT1003.619789E-1034.49999669
Produced by FastQC (version 0.11.2)