FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n02_zt14ss31.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n02_zt14ss31.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences243496
Sequences flagged as poor quality0
Sequence length75
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG115624.748332621480435No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAATCG150.002353978368.995074
GGTACAA200.00734821151.75693524
TATACCG200.00734821151.7569355
CTAATAT200.00734821151.7569351
CTTAACG200.00735418351.746341
TAAGACA200.00735418351.74634
GAGTACT306.2974467E-446.00616516
TGCGAGT350.001344841839.43385313
ATTAGGT350.001346200939.4257553
GTACAGA400.002590630734.5046231
GCGAGTA400.002590630734.50462314
TAATATC400.002593242334.4975362
TATCCGG501.8579036E-434.49753647
GTTCTAA553.246997E-431.3678361
TACACCG553.246997E-431.3678365
GCTTATA553.2509124E-431.3613952
TCTAATC553.2509124E-431.3613953
ATGCGTT450.004617225430.6644744
GTCTTGG854.4047392E-628.4155711
ACTCGCT500.007718096527.6036977
Produced by FastQC (version 0.11.2)