FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n02_zt14ss30.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n02_zt14ss30.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2161869
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG31800.14709494423575156No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCTA5050.029.37611810
GCGCTAT5300.028.64139411
GCTATCG5400.028.11099813
TTGTGCG5900.025.728717
CGCTATC6050.024.52056112
TATCGGT6050.024.52056115
CTATCGG6350.023.90541614
ATCGGTC6700.021.62677816
TCGGTCT6850.021.153217
TGTGCGC7200.021.083258
CGATCTG66850.020.8495795
CCGTCCG6950.020.8488379
ACACCGT8100.020.0184386
CGATCTT35850.019.9204225
GTGCGCT7450.019.912679
GATCTGG22850.019.3259626
CTAGTCG7550.019.19197720
ACCGTCC7400.019.1147888
AACACCG8500.019.0763935
CCGATCT174550.019.053494
Produced by FastQC (version 0.11.2)