FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n02_zt14ss2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n02_zt14ss2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2031113
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG33130.16311253977499035No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTCG658.65977E-426.53826347
CGATCTG53600.024.523745
CCGATCT157550.021.5253854
CGATCTT52800.021.1054745
TTCCGAT162550.021.0123542
GATCTTT42600.020.5707766
TCCGATC166000.020.5335833
TCCGACC850.004121433520.29396635
TCTGCGC3100.020.0326028
TTTCGGA4150.019.952641
GATCTGG22700.019.7580476
ATCTGCG6500.019.6387987
CTTGTCG1108.2373805E-418.81896821
GCCGGGA4500.018.40076813
CGTAACG950.007837919518.15731253
CTTCCGA192400.018.0576671
TTCGGAA4400.018.03442
TCGGGTA1150.001110311118.00075125
CGTAGGG1804.1987023E-617.2502962
ATAGTGT17550.017.10201669
Produced by FastQC (version 0.11.2)