FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n02_zt14ss29.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n02_zt14ss29.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences323279
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5090.15744913836036364No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGAAT150.002354011669.011
TGTACGA200.007354275351.752
CCAAGCG350.001346382339.4285743
ACGACTA400.00259358734.560
TGATCGG500.007726832327.65
ATCTACG500.007726832327.67
TCGCTCT500.007726832327.616
TTATACT1251.2586679E-724.844
CGATCTT6250.024.2885
ATTATAC1301.8388528E-723.8846153
TATACTG1452.0036168E-823.7931025
CTTATAT1352.6469934E-723.03
AAGGGTG750.001989942723.05
ACAATGC1052.2742237E-522.9999988
CGATACT4400.021.95454669
GTATACT953.0590303E-421.7894744
GACGAGG953.0590303E-421.78947468
GTAGGAC800.002894975221.56253
TAAGGGT800.002894975221.56254
TAGTACT800.002894975221.56254
Produced by FastQC (version 0.11.2)