FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n02_zt14ss28.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n02_zt14ss28.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2089801
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG32900.15743125780875786No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGTA951.0512649E-525.42117720
GGCGCGT1258.7450586E-519.32009319
CGATCTG76950.018.5162165
CAACGCG950.00783740418.15754956
CGATCTT40500.018.0589145
TAGACGT9950.017.3363534
TACGACG1452.7241505E-416.6548545
CGGTCCA1250.001941575316.55968510
CCGATCT198850.016.5166574
TCTGTCG5050.016.3957278
GATCTTT27400.016.3683016
GTATTAA7000.016.2651421
ATCCGCA1503.527366E-416.09969350
ATCTGCG6250.016.0076947
TACCCCG1300.00252295915.92277210
TTACGAC1300.00252295915.9227724
CTAGACG8300.015.7948793
TTCCGAT211350.015.752762
ATCTCGC6800.015.7276417
TCCGATC213550.015.6220393
Produced by FastQC (version 0.11.2)