FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n02_zt14ss26.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n02_zt14ss26.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2533134
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG28410.11215356155655405No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG81600.019.491265
CGATCTT37300.018.869075
GATCTGG25650.018.6963396
GTTCGGC950.00783772518.15759811
TAACCGT1200.001477212917.25046
CCGATCT209950.016.5971954
ATCTGCG6600.016.204927
TTCCGAT218250.015.98181252
TCTGTCG6750.015.8448128
TCCGATC221350.015.8203343
CTGTCGC5700.015.7372079
CTGGTCG2201.6219965E-615.6821829
ACGGTTG1554.527357E-415.58039269
CTTCCGA230850.015.4093271
CTGTGCG2705.254151E-815.333699
GTATTAG8600.015.2454421
TGTCGCC5250.015.11403910
TGGTCGG1851.0222268E-414.91867510
ACTGTCG2152.264489E-514.4421968
TACGGTT1450.005217745414.27562968
Produced by FastQC (version 0.11.2)