FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n02_zt14ss25.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n02_zt14ss25.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10817543
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG172700.1596480827485502No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTT170750.019.7201255
CGATCTG385600.017.4295
TAGACGT42550.016.054114
CCGATCT977800.015.97284
CTAGACG31300.015.6518113
TTCCGAT1026650.015.3038692
GATCTGG125500.015.20204456
TCCGATC1037900.015.1442863
GATCTTT139900.015.1415826
CTTCCGA1082200.014.8219981
GTATTAT56000.014.4788671
GGGGTGC69650.014.2161286
CGAATAT19650.013.87014869
GTATTAG44750.013.5698281
TAATACT47050.013.5654034
ATAGTCG9700.013.51538568
CGAATAG225400.013.46930969
GTATAAG39750.013.1067351
ACGTGCG11200.012.93760516
GTATATA100350.012.9278161
Produced by FastQC (version 0.11.2)