FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n02_zt14ss24.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n02_zt14ss24.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences94442
Sequences flagged as poor quality0
Sequence length75
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG73657.798437136019991No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATCTC150.00234883969.016
CCTACTT150.00234883969.06
ATTTTAC150.00234883969.015
TACTAAC150.00234883969.07
GTGACTC150.00234883969.049
CATATCC252.555096E-455.217
CTTATTT252.555096E-455.212
TGTCATG200.00733823951.75000463
CCCCTCG200.00733823951.75000467
CCCTCGC200.00733823951.75000468
CTACTGC200.00733823951.75000413
CGGTTAT200.00733823951.75000439
GGATCGG200.00733823951.7500041
GGTTTGT200.00733823951.7500046
TATATAC200.00733823951.7500043
TGCATTG200.00733823951.7500046
GAAAATT200.00733823951.75000435
CGGGAAT200.00733823951.75000451
AACTGAG200.00733823951.75000431
ACCACTC200.00733823951.7500041
Produced by FastQC (version 0.11.2)