FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n02_zt14ss23.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n02_zt14ss23.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61314
Sequences flagged as poor quality0
Sequence length75
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG756512.338128323058354No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACTA150.002344898269.011
TCCGCTT150.002344898269.063
TAGGTGT150.002344898269.069
GAACTAC150.002344898269.012
CCGCTTA150.002344898269.064
TATCTCT150.002344898269.023
ACTACAG150.002344898269.014
TAAGCAA150.002344898269.024
CTTAGGT150.002344898269.067
TGTAACC150.002344898269.024
AGTACTC150.002344898269.053
GTAACCC150.002344898269.025
TCGAACT150.002344898269.010
TTCTCCG150.002344898269.060
TTAGGTG200.00732602451.7568
CTATGAC200.00732602451.7569
CTCGTTC200.00732602451.7525
CTGTAAC200.00732602451.7523
AACCCCT200.00732602451.7527
TCCTCCT200.00732602451.7534
Produced by FastQC (version 0.11.2)