FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n02_zt14ss22.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n02_zt14ss22.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1929571
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30510.1581180480013433No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTT30700.022.1383365
CGATCTG65800.020.238515
GATCTGG23600.019.8812666
GCCGACC1751.474109E-719.71419569
CCGATCT168600.019.0506274
TTCCGAT174600.018.455722
TCCGATC177750.018.128193
CTTCCGA186000.017.6598151
TAGGTGT13650.017.43948269
ATCTCGC6850.017.124017
ATCTGCG5650.017.097277
CCGAAAA10950.016.06883817
CTGTGCG2603.3040124E-815.9230059
TAGACCG1756.360434E-515.7713575
AGACCGT2201.6219765E-615.6817476
GATCTTT26100.015.5976316
ACCGTTA1554.5262557E-415.5805748
ACGGCGT1350.003244205815.3332646
CCGTTAT1350.003244205815.3332649
TTAGGTG16450.015.10023669
Produced by FastQC (version 0.11.2)