FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n02_zt14ss21.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n02_zt14ss21.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1404430
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG27200.1936728779647259No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACGGT658.6581084E-426.53843543
CGTTGTC851.443532E-424.35291741
GCACCGT1004.335494E-420.699986
TTTCGGA1651.828339E-618.8188321
CGATCTG43900.018.3895035
TTCGGAA1702.4322508E-618.2653372
CGATCTT19900.018.2034995
GTGTATA7000.017.743471
CCTAGTA2802.5465852E-1017.2505972
GATCTGG14100.016.8829616
CCGATCT112000.015.9562334
ACATCGC1300.002522101915.923068
CGACGAG1756.358271E-515.77141266
GTATAAG4650.015.5811831
TTATACT5350.015.476624
GTATGGG3805.456968E-1215.4347461
CGATACT33100.015.42596669
TATACTT5600.015.4017715
TTCCGAT119400.015.3723912
GTATAAA7250.015.2281111
Produced by FastQC (version 0.11.2)