FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n02_zt14ss20.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n02_zt14ss20.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2194615
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG31710.14449003583772096No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATATA13500.018.4007361
GCGGTAA2501.0513759E-917.93949154
ATCCCGC1200.00147696217.2506896
CCGCGGT2802.5465852E-1017.24951252
CGCGGTA2601.7716957E-917.2495153
CGATCTG75800.016.977595
ACGTTCG1855.4482116E-616.78483624
TAGACGT9950.016.643884
GGACCGT2301.423814E-716.5006586
ATCTGCG6900.016.5006587
CGATCTT38550.016.2885885
CCGATCT193700.016.1552664
GTTCGTA1958.9844525E-615.92407626
TACGACG2654.1745807E-815.6232665
TTCCGAT203750.015.4765842
CGATATG57700.015.36611569
TCCGATC207350.015.2082273
GATCTGG23750.015.1079726
CTAGACG7850.014.9432733
CTTCCGA214950.014.9112721
Produced by FastQC (version 0.11.2)