FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n02_zt14ss19.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n02_zt14ss19.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1303220
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20650.1584536762787557No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTTCA658.6578034E-426.53841857
CGTGTAG2300.025.49995869
CGATCTT18350.022.185255
TACGCCT800.002899665921.56246610
ATCTGCG2303.45608E-1019.4999687
TTGCGGC1451.2650331E-519.0344511
CGATCTG38900.018.3585825
TACTTCG950.00783507618.1578647
CGCATTT950.00783507618.15786446
ACGGCGA950.00783507618.15786463
GATCTGG12350.017.8785116
ATTATAC5500.017.5636063
TCTGCGC1402.0828746E-417.249978
CCGATCT97750.017.1882084
TATACTT5250.017.0856865
TACGTGT5700.016.9473469
TTACGTG5950.016.81509868
CCGACGC1855.4481316E-616.78375669
TAGACGT3309.094947E-1216.7272454
GCCGACT1653.8422837E-516.72724567
Produced by FastQC (version 0.11.2)