FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n02_zt14ss18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n02_zt14ss18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2274250
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG28280.12434868638012532No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAACG851.4441276E-424.3523953
TCCGACC1253.5064168E-622.079535
TAGACCG800.002899936921.562965
AGCGCGT1206.3917105E-520.12454410
TGGCGTA1751.4746729E-719.71383950
CGATCTG74150.019.5418875
TACACCG2354.6020432E-1019.0855145
TTGTCGG1651.8292012E-618.81899822
CGAATCG950.00783777218.15748259
GCGTAAC950.00783777218.15748252
GATCTGG25600.017.924216
ATTCGAC1200.001476950817.25074821
CGATCTT32900.016.6736075
CGCAATC1250.001941692816.55962635
GCGCCGG2301.4248872E-716.49962611
CCGATCT192250.016.492164
TGTCCCG1907.026565E-616.34173410
TTCCGAT201050.015.8900672
TCCGATC203750.015.6629113
TAATCCG2001.1431834E-515.5246548
Produced by FastQC (version 0.11.2)