FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n02_zt14ss16.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n02_zt14ss16.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences129856
Sequences flagged as poor quality0
Sequence length75
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1718313.232349679645145No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1860.14323558403154263No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTAA150.002347606569.0238914
CTCGTTA150.002347606569.0238913
AGAACAT252.5591813E-455.19784567
GAGCTCT200.00734555651.7479811
GTTTCCG200.00734555651.7479832
ACACAAT200.00734555651.7479837
CAATTGT200.00734555651.7479864
ACCCTCT200.00734555651.7479861
CGTTATG200.00734555651.747985
CGCTACT200.00734555651.7479863
TACTAAT200.00734555651.7479829
TGCTAGA200.00734555651.7479851
GTCCAAT200.00734555651.747982
CTGATAT200.00734555651.7479844
TTGGGCG200.00734555651.7479855
GTTTTGG404.9785936E-543.1233141
TATTCAC350.001343669139.4270322
GAGCCGT350.001343669139.42703262
CCTATTG350.001343669139.4270323
ATATCTG501.8527894E-434.49865344
Produced by FastQC (version 0.11.2)