FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n02_zt14ss15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n02_zt14ss15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1696351
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG25050.14766991029568763No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT553.2579954E-431.3631136
CGGTGTA5250.022.99961769
ACGGTGT8000.021.130969
TACTCGC850.004121375720.2937869
CGATCTG64850.020.002755
CGATCTT31950.019.3283185
GATCTGG20300.018.3543746
TACGTCC950.00783670318.157596
CCGATCT163800.017.7341374
TACGCTC2401.2312739E-817.24971461
AGCGAGT1200.001477334817.24971465
TTCCGAT171750.017.2160842
ATCTGCG5900.016.9573467
TCCGATC173400.016.931383
CTTCCGA181500.016.5968721
GGTCCGG1250.001940958116.56021311
GTATTAG5300.016.2761941
GTATAAG6050.015.9695011
GACGGTG10550.015.69642168
TATACTG7550.015.5361655
Produced by FastQC (version 0.11.2)