FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n02_zt14ss14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n02_zt14ss14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2881597
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG45140.15664924692800555No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG107700.019.4125635
CGATCTT52300.018.4046885
GATCTGG35150.018.2563366
ATCTGCG9000.017.6336147
CCGATCT269200.017.4293944
TCCGTAA3003.6379788E-1117.24967450
TTCCGAT285200.016.5002612
TCCGATC287000.016.4926593
GTATTAG10950.016.0693051
GTATCGA3451.8189894E-1116.0002549
GTCGCGG1300.002522974315.92305311
GCGACCC1958.993369E-615.92277759
CTTCCGA302000.015.7999711
TCTGTCG5700.015.7370918
ACGCGCT2201.622604E-615.68179447
GCTCCGT3101.0841177E-915.58062148
GATCTTT33000.015.3684246
TTATACT11050.015.2986194
GGTATCG4101.8189894E-1215.1465828
ATCCGCG4001.2732926E-1114.662477537
Produced by FastQC (version 0.11.2)