FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n02_zt14ss13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n02_zt14ss13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2266004
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG36110.15935541155267158No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG75300.019.15115
CGATCTT36700.018.7067995
CGATACA2801.2732926E-1118.4818669
TCGCGTC1351.5774451E-417.88940430
CCGATCT190800.017.5209564
ATCTGCG7200.017.2497377
TTCCGAT199550.016.9097772
GATCTGG25600.016.8454446
ATACCGT2057.7634104E-716.8290086
TTTCGGA2502.035631E-816.561941
TCCGATC203850.016.5346983
CTTCCGA210600.016.2689971
GTTCGAA1704.964956E-516.23504469
TAGGCGT1704.964956E-516.2350444
GTATTAT9000.016.1018851
TAACCGC3058.60382E-1015.83582368
GTAGGAC3753.6379788E-1215.639763
TAATACT9200.015.3747644
TATTTCG1605.7618E-415.0935186
GCGTCGT1605.7618E-415.09351832
Produced by FastQC (version 0.11.2)