FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n02_zt14ss12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n02_zt14ss12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1379529
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26490.19202205970298558No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCGTT200.007360108751.7486456
TACACCG1304.922571E-621.230985
GCCGTGC1056.023422E-419.7144838
CTGTACG900.005734655519.1668579
AGTGCCG1451.2653891E-519.03398310
ATCTCGC5750.018.6001857
ATCTGCG4650.018.5485737
TAACGCT950.00783477518.1580754
GCACTGA8250.017.9819986
ATAATAC5950.017.974973
GTGCCGT1552.2470738E-517.80598311
ATACGTC1200.001476882317.2501725
TGCACTG10850.016.8527035
CGATCTT26000.016.852095
GATCTGG16900.016.7398136
CGATATG31900.016.65473669
TCTCGCC3800.016.3422688
GTGCCGA1704.9631555E-516.23486966
CGATCTG55900.016.0466735
GTATTAT6150.015.70861151
Produced by FastQC (version 0.11.2)