FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n02_zt14ss11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n02_zt14ss11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1286177
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG28630.2225976673506057No Hit
GTTTATGGTTAAGACTACGACGGTATCTGATCGTCTTCGATCCCCTAACTTTCGTTCCCTGATTAATGAAAACAT15100.1174021926997606No Hit
GAATAATAGAATAGGACGTGTGGTTCTATTTTGTTGGTTTCTAGGACCGCCGTAATGATTAATAGGGATAGTCGG14670.11405895145069457No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTATC2100.029.57126212
TGCGCTA2200.028.22711410
GCGCTAT2300.026.9998511
CAGCGTA658.6579454E-426.5383119
TTGTGCG2500.026.2198527
CGCGCAT1101.1530155E-625.09076740
GCTATCG2500.024.8398613
AACGCAT1151.697992E-623.9998666
CGATCTG32650.023.563425
TAACGCA1202.458337E-622.9998725
GGTTCGC1052.2811519E-522.9998710
ATCTGCG3350.021.6267457
ACGTTCA1605.7176294E-821.56321526
TCTGCGC2101.0186341E-1021.3570238
ACGCATC1154.603379E-520.9998847
CGCATCA1154.603379E-520.9998848
CCGATCT76350.020.876114
TCCGGCC3000.020.7006923
TCGGGTC2501.8189894E-1220.7006924
TATCGGT3100.020.03214515
Produced by FastQC (version 0.11.2)