FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n02_zt14ss10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n02_zt14ss10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1749068
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22340.1277251656310675No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCGA500.007736160427.5998846
CGTACCC2207.2759576E-1221.95445410
TAACGGC800.002900100521.562414
CGACATA850.004121127520.29403340
CGATCTT27650.019.3398865
CGATCTG61650.019.1945695
CGTCCGG1301.1815114E-418.57684511
GTCCGGG2307.2723196E-917.99992612
TATCGCC1150.001110568917.99992622
ACCGTAC2501.0513759E-917.9399268
CCGATCT152250.017.4482024
TTCCGAT161300.016.5348262
TCCGATC162700.016.4335523
GATCTGG20350.016.1055856
GTACTAA4500.016.1031551
TACACCG1503.526555E-416.0999345
GATCTTT17600.016.0737976
TCGCCTA1300.002522486715.9230124
TTGTCGG1300.002522486715.9230122
CTTCCGA170750.015.8640171
Produced by FastQC (version 0.11.2)