FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n01_zt14ss9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n01_zt14ss9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3437212
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG7150.030.39851843
TGCCGTC7150.029.43348545
GCCGTCT7650.027.9607146
ATGCCGT8950.024.67032444
CGTATGC8700.024.58613841
TCGTATG9000.024.14993340
CCGTCTT8600.024.06970247
CTCGTAT9200.021.74993939
CGATCTT55450.020.905875
GTATGCC11800.020.17367442
ACCGTTA12300.018.7929048
ACTCGCG2801.2732926E-1118.48209457
CGTCTTC10750.018.29297348
CGATCTG114100.017.779615
GATCTTT38500.017.3848746
GACCGTT13500.017.3779837
ACGGCCG13250.017.18485820
GGACCGT15350.017.0818826
CGCCGTT13150.017.05318525
CCGATCT279850.017.0008074
Produced by FastQC (version 0.11.2)