FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n01_zt14ss95.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n01_zt14ss95.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29781
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG890.29884825895705314TruSeq Adapter, Index 1 (96% over 29bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGGCT150.002333002969.0000146
GTGTGCT150.002333002969.0000152
AGTGACT150.002333002969.0000124
GGTGATT150.002333002969.0000141
CGCGTTT150.002333002969.0000139
TTCTGGG150.002333002969.0000152
TCTGGTA150.002333002969.0000138
TCGCGTT150.002333002969.0000138
GGGATGC150.002333002969.0000142
GCGTTTG208.3788604E-569.040
CGTGTGC208.3788604E-569.051
GGTTGCC208.3788604E-569.042
GTTGCCG208.3788604E-569.043
CGTGAAA501.4551915E-1162.10000257
TGCCGTG454.129106E-1061.33333245
CGTATGC1250.060.7241
TGCGTGA401.1434167E-860.37500455
GCCGTGT353.178884E-759.14285746
GTATGCC1300.058.38461742
ATGCCGT950.058.10526344
Produced by FastQC (version 0.11.2)