FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n01_zt14ss94.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n01_zt14ss94.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2173342
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG37350.028.6351415
CCGATCT96700.027.6862074
GATCTGG19450.027.494156
TTCCGAT99050.026.8900152
TCCGATC99650.026.7973523
CTTCCGA108600.025.0336741
CTGCGCG2053.6379788E-1223.5609269
TCTTCCG70450.022.6250325
TCTGCGC3100.021.1451198
ATCTGCG6200.021.1451197
CGATCTT52000.020.833135
TGCGCGG2201.9099389E-1020.38632210
GCTCTTC79250.020.373933
GTGCTCT78700.020.0340941
GCGGGGA3550.019.43657913
TGCTCTT83700.019.3319472
GATCTTT52450.018.5494846
CTCTTCC88200.018.4238574
ACCGGTA3153.6379788E-1217.52377315
TGTACCG3950.017.4687215
Produced by FastQC (version 0.11.2)