FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n01_zt14ss93.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n01_zt14ss93.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3459852
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTT59000.018.8286915
CGATCTG113550.017.7132265
GATCTTT39400.017.5125756
AGTTCCG9100.017.06032832
ACGAATG8500.016.23518845
CCGATCT298850.015.9425114
TGGCGTA7450.015.74486450
CGCCGTT17600.015.68171625
TTCCGAT309100.015.5591662
ACCGTTA18850.015.3739058
CACGAAT9000.015.33323544
TCCGATC314850.015.2857513
GCGTAAC7700.015.23366752
TCCGACC10250.015.14668135
ATCGCGT2051.4441266E-515.14668137
TACACCG2756.585651E-815.0544485
CTTCCGA325200.015.0328681
GGACCGT19750.015.0226866
ATAATAC13450.014.8772273
TTGTCGG9750.014.86144222
Produced by FastQC (version 0.11.2)