FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n01_zt14ss92.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n01_zt14ss92.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1946122
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGCGT800.00290014721.562537
TAGACCG1056.025046E-419.7142875
GACGCCC1451.2655393E-519.03448156
CGATCTT35050.018.8987145
CGAGTCG950.00783635418.15789667
GACCGTT1150.001110599418.058
CAATACG1150.001110599418.034
CGATCTG66250.017.5494335
TCGCTTA1200.001477275517.25000228
GATCTGG21450.016.8881136
GGGCTCG2151.2762812E-616.04651354
GATCTTT26900.016.0315996
TCTGTCG4100.015.9878038
GTCGTGT1554.5261238E-415.58064668
CCGATCT177850.015.4023064
CTGGTCG1350.003244121615.3333339
TTCCGAT183700.014.9493742
TACACCG1400.004132207514.7857135
TTATACT9150.014.7049184
GCGTCCG1657.274727E-414.63636348
Produced by FastQC (version 0.11.2)