FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n01_zt14ss91.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n01_zt14ss91.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1999345
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGCGT1202.6884663E-928.74997937
TATGGCG1001.5644227E-524.14998443
CGATCTT38950.021.2580095
TGCCGTC1657.9238816E-820.90907745
CGCGTAT1004.3363712E-420.69998639
CCGTTTA5500.020.69998627
TCGCGTA1258.745195E-519.31998638
ACGGCCG6150.019.07315620
CCGTTAT6250.018.7679869
ACCGTTA6650.018.676688
TACGGCC6300.018.61903419
CGCCGTT6500.018.5769125
GATCTTT30200.018.2781316
GACCGTT6450.018.1860337
CCGCCGT6700.018.02237524
GGACCGT6600.017.7727156
GCCGTTT6850.017.62772426
TAATACT9600.017.2499894
TATGCCG1653.8439066E-516.7272643
GATCGCG3157.6397555E-1116.42855836
Produced by FastQC (version 0.11.2)