FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n01_zt14ss90.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n01_zt14ss90.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1006916
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACCGC1953.8198777E-1122.99973768
GTTCGAT1653.1432137E-922.99973543
GACGGTC1103.2666838E-521.9542928
ACCGGTA953.0654945E-421.78922315
GATCTTT17600.021.5622526
CGATCTT22300.020.885415
ATATCGG1004.333176E-420.70078839
ACCGTTA1507.3311094E-720.699768
TAAGTCG850.004120107720.29388444
CGTCGTA1056.022745E-419.71405833
TGCAACG1751.4724719E-719.71405819
GGTAACG1601.3616263E-619.40602726
CCGATAT1258.7404245E-519.31977733
TCGCGTC900.00573431519.16644530
TTAACCG2354.5838533E-1019.08583567
ATCTTTT19400.018.85037
GGGTTCG1651.8272294E-618.818941
TAACGGG1651.8280971E-618.81796528
CGCCGTT1651.8280971E-618.81796525
TCGATTC1852.6425369E-718.64843645
Produced by FastQC (version 0.11.2)