FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n01_zt14ss8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n01_zt14ss8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences101
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGCCTTCAGATCCGGAGCCCATCGATGACTTGCTCTCTGACTTTTCCAATTGGGGTGCCTTCTGTCTCTTATA10.9900990099009901No Hit
GTCGTACGGGATCCCGGCTTTACCTAAGGTGAAAATAATAGGAGCCTTTTCCCATATCTCTGTTTGCTCAAGAAC10.9900990099009901No Hit
GGGGAGGGGGGAGGGATAGCATCGGGAGATATACCTAATGCTAGATCTGTCTCTTATACACATCTCCGAGCCCAC10.9900990099009901No Hit
GTGAGGGCCTCGTCGATCTGGCCTCCCTCAAACCGCCGGGACTGCGCCGACACGACCGAGCGTTCCGCCGTAAGC10.9900990099009901No Hit
ACCTTAAACTCTGACCGCCAGTGAGCCGGGCAGAACAGAGCCATATTTCTCTTCTTTCAAAAGCAAATGGAAGAA10.9900990099009901No Hit
GCTCCCGGCCGGTACGCGCGCGCAGGTCGCACGCATACTGTCTCTAATACACATCTACGAGCCACTAGACGTAGC10.9900990099009901No Hit
GATCTGATCAGGACAGGCGGCCTCGAGGCCGGAGTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGG10.9900990099009901No Hit
GGCTTTTCTGTCAGCTCGAACGTCTCGATGGCCCGCGAGAAACCGTAATACAGGCCGTGCCAGAGGTTGGCTGTC10.9900990099009901No Hit
GTTTTGAAACGCGATACGGCAGAGGCGATGACCCACTCTGTGAGCGGGTGAATCAGATCGCTGACTTCAACCACT10.9900990099009901No Hit
GTAGATATCTGCAGGATTATGGTGCTGCAGCTTATGTAGCCGTCATTCATGATGGCAGTAAAATGCTTATTGTGT10.9900990099009901No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCGGGGGTGGCGGGGTGGGGGTGTAAAAAAAAGGGGGG10.9900990099009901No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAAAAAAAA10.9900990099009901No Hit
GTCAGGAGATCGAGACCATCCTAGCTAACATGGTGAAACCCCGTCTCTACTAAAAATACAAAACATTAGCCGGGC10.9900990099009901No Hit
CACCGATGGAGTCCATAAATGACTTGGAAAACTGCACCATGGGCACCTTCACGTGGTAGACGCTCGTTCCTCCGA10.9900990099009901No Hit
GGGTATATACCTAGTAATGGGATTGCTGAATCGAATGATATTTCTGTCTTTAGGTCTTTGAGGAATTGCCACACT10.9900990099009901No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCGCGGTTGCCGGCGTGGGGGTTTAAAAAAAGTGGGGG10.9900990099009901No Hit
ACCGCTCGTTCCGGGCCGGTGCGGAGCAGTCGTCGTTCACGCTGGATTCGGACTGTCTCTTATACACATCTCCGA10.9900990099009901No Hit
CCGCTTCAATGATGCCCAGCATCAGGTCGCGCCCGTCGGACGTGGCCGTGATGTCGAGGAAGGTGATTTCGTCGG10.9900990099009901No Hit
GTTGATGACAGTGCGAATCGGCTTGCCGGTGACTCCCTTCCACTCACCCGAACGCACCTTATCCGCAAAGGCGTA10.9900990099009901No Hit
GTAGAGGGGCAGCAGGAGGATGCACATGCGTGCTTCAGTTACCGATTCTAGATAATGACTGCACAAAACTACACA10.9900990099009901No Hit
TCGGTTCGGTGATCGCCTTTACTGGCGTGGTTCTGGCAAAAGTGATTGGCGATTTCGGCCTCTCGCCGCTACTGG10.9900990099009901No Hit
TATTTCATTGATCGGAAGCCCTAACTCCTCACAAATTGCGTATGCCACTTGAAAAGAGGGCAGCGATCGGTTATT10.9900990099009901No Hit
ACAAAAGTGTTGTAACTCTTGCCTAGGATTTGCCTACAGGGGACATTATGACATATCTCTGCACTGATCACCCAG10.9900990099009901No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATGGGGGTTTGGGGGTTGGGGGTTGAAAAAAAATCGGGG10.9900990099009901No Hit
TAGCTGCATAAGCAGGCAGTAATTGTTCGCAAAGTGAACTAATTGGCATTCAAAGGATATCAGCCAGGCGACTGT10.9900990099009901No Hit
TATTGGAAGTATGATCTCATTCATTGCTGTATTCGTATTTGCTTTCACAGTATACGATGCACTAGCAAATGGAGA10.9900990099009901No Hit
GCCGCCGGCTCGTCGATCAGCAGGACCGTAGGCCGCGCGGCGAAGGCGGCGGCGATCTCGATCTGGCGCTGCTTG10.9900990099009901No Hit
ACCGTGAAGGCATCGCTGTCGGTCGAGATCGAGAACGTCCAGCCCATGGGTTCGGCAGCGAACTGCTGGAACGTG10.9900990099009901No Hit
TTTCATGAACATAGATGCAAAAATACTCAACAAAATACTAGCTGTCTCTTATACACATCTCCGAGCCCACGAGAC10.9900990099009901No Hit
GGTCAAATTATCCACGTGCAGACTTTACAAACAGAGTGTTTCCAAACTGCTGAATGAAAAGAAAAGTTAAACTCT10.9900990099009901No Hit
GTAAGGGACAAAAGAAGACTCAGAGCCATCGTGAGCTTGTCTCCCAAAGCTCAAGAAAGCTGTGCAGGGTGGATG10.9900990099009901No Hit
GACTCGCCATACGTGAACTTCCTGGTCGCCCGCCCGGACAACAAGGACAGCGAAGCCATCCAGAAACTGTCTCTT10.9900990099009901No Hit
AAGTGTGGCGTGTTCACACGACAAAATGCTGCACAGCGATGAAAACGCCCGGCAGGCAGCTACACGCAGCGGTAG10.9900990099009901No Hit
TCGGCAAATGGCACGCACTGTACGACGCGGCCCAGCACATCGCGTAACGACGCGGGATAATCGATCGGCACCGGC10.9900990099009901No Hit
GAAATACACCGGGTGCTCGCGTCGCTCGATGATGTCGGGGCGCTCCAGGGAGCGCGCCAGGGTCTGTCTCTTATA10.9900990099009901No Hit
GGTCGGCACGTATATTCTCGAACATGGCTCCACCTACCTTGTCGGTTGGCTGGCGTACCGGGTCTTGAACAACAG10.9900990099009901No Hit
TTTGTACTCTTAGTAGAGACGGGTTTCTCCATGTTGGCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTA10.9900990099009901No Hit
TCATAGACCTTGACCATCTGTTCGAGTTGATCTTTGCGGGAGCTGACCTCGGACAAGCTCATGTCGGCTTTCGCC10.9900990099009901No Hit
CCGACATACCCCGAACACCCGCGCCGCCTGCTGATGCGCTGGCGCTCCCAGCCGATAACCTGTCTCTTATACACA10.9900990099009901No Hit
GTGCAGCGACAAGCTTGCCGCCACTCGCGTTGGCAATCGAACCCGTGCTGTTGCCGATCAGGTTGCCGGTGTTGG10.9900990099009901No Hit
GCTCTTTGCCACCACGTCCTGCATGCCCAGCGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGC10.9900990099009901No Hit
GACCATGAACAATGGAAGGACGAAGGGGTTGCAATAAAATGGTGGCTGCGACTGTTTAATAAAAACACAGCTCTC10.9900990099009901No Hit
GGACTGGGGACTGAAGATTTTCCCGGCGCGAGAAAGCGAAAAGCCCCGGTGCTGTCTCTTATACACATCTCCGAG10.9900990099009901No Hit
ATTCAGCATAATGTTGACTGTGGGTTTGTCTTTTATTGGCTTTTATTACATTAAGGTATGTCCCCTTTATGCCAA10.9900990099009901No Hit
GCCAATATGAGCCCATTGCTCCTATCTGAAGACAGAATCAAAATACCAACTCCCGCAGCAACGCCCCTCACTAAA10.9900990099009901No Hit
GTTACAACACTTTTGTCATCAGTTCACGGATATGTAAAAACGACCCCGTAGGCAGCAGTCTCTTATACACATCTC10.9900990099009901No Hit
CACTGAATGGACCCGGATTTAAACGAAACCGCCAGCCAAGGAGGTTGACCTCGCGATCCGGCAGTAGGCGTGCAA10.9900990099009901No Hit
AGTCAAGTTTCTCCATAGGGACAGAACTAATAGGATACGTGTATATGCTGTCTCTTATACACATCTCCGAGCCCA10.9900990099009901No Hit
ACCCAAAGCAACCTACAAATTTAATGCAATATCTACCAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTA10.9900990099009901No Hit
AATTAAAAAAACCTACGAGACACCGTGACTAGTCATTATCCTGTATCACTTATACTCATTTAAAATCAAAAAGGT10.9900990099009901No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATGTGGGATGCCGTGGTATTGGTTTAAAAAAAAAGGCGG10.9900990099009901No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCGGGTTTTGCGTGTTGTGGTTGTAAAAAAAAAGGG10.9900990099009901No Hit
ATCTTTCAGCGCTATCCATTTTGACTTCTGCGCATTCGCAGCCGCCTGGCAGGTCCCGTACCTCCACCGATTCAC10.9900990099009901No Hit
CGTTGGCGATGATTACCTCGTGCCGCGCCGCACCCAGCGCGCGCAGATATTCGCGTTCGATCGCGCGTCGGTTAC10.9900990099009901No Hit
ATGCAGACCACACCAGTCCTCATTCAGTAGATCTTACAGGAAGTAGAAGTAGCTGTCTCTAAAACACAACTCCGA10.9900990099009901No Hit
TCAGAAGTGCGTGCGCACCTTAGACATTGATGAGGTTGGCAAGACCACTCGTCACGGCACTTTCTTCCAGATGTT10.9900990099009901No Hit
AGTTGAAGCTAGCGTGACTGAAGGTGCCATTCGTTTGGGGCTTGGCTCAGTCAAAGGCTTGTCGACGAAAACCGC10.9900990099009901No Hit
GCTAGGCGAAGCGGTGGAGTGCTGCACCCTAGATGGCGAGAGTCCAGTAGCCGAAAGCTTCACTAGCTTACGCTC10.9900990099009901No Hit
CCCTTGCCCCATGCCGGGCAGGTCATCGGGGCGATAGGCGTGTCTGGTGCCAGCGAGGCCATCGACACACTTTGT10.9900990099009901No Hit
CATTAGGGAGGGCAGTCTGCTTCACTCAGCCAACCACTTCAAACATTACCACTTCAAATATCAACCTCATCCTGA10.9900990099009901No Hit
TCTCGCGCGGCCCTGGCGCCGTTCGTCGCCTGTCTCGTATACACATCTCCGAGCCCACGAGACGTAGAGGAAGCG10.9900990099009901No Hit
AGTAAAGGATAGGGATTTTCTTTACTATTCACTTTCATTTAAATTGTTCACAACCCCTCTGTACCAGGGAGACAT10.9900990099009901No Hit
CCTTATGGTTTTATAAGCATCTGGTATTTCCCCTGCTAGCACTTCTCTCTCCTGCTGCCTAGTGAAGAAGGCTCT10.9900990099009901No Hit
GCTATACGAGCGAGTTTATCATGACAAACAGCGGAGGAAAAGAGACAGGCGTTCAAGTTTCCATCATCGAAAAAA10.9900990099009901No Hit
CTCCCTAACTCATTTAATGAGGCAAGCATTATCCTGATACCCAAGCCCTGTCTCTTATACACATCTCCGAGCCCA10.9900990099009901No Hit
GTGAGGATCACGATGAGCCCATCTGCCTCATCTGCAGTCTGAGTCAGGAGCACCAAGGCCACCGGGTGCGCCCCA10.9900990099009901No Hit
ACGACCGCGTGCTCGGCGTGGTGGAGCTGAAGGACATCGTGAAGGCCGGCATTCGCGAGCGCTTTGCCGAGCTGC10.9900990099009901No Hit
ATGCTTGGCTGTGCGAACAACTGTTCGGCAGCCTCAGGCCGTTCAAGTTGCCGGCAGGGATGGTTTTGCTGAATG10.9900990099009901No Hit
GTGCAGCGACAAGCTTGCCGCCACTCGCGTTGGCAATCGAACCCGTGCTGTTGACGATCAGGTTGCCGGTGTTGG10.9900990099009901No Hit
TAGTGTTCCAATTTCGCGGCGATCCTTGCTCTTCACCGCAGGGTCGATTGCCCTCACCATTCCGCTGCGGCGCGC10.9900990099009901No Hit
GAATGGAATCATCTTCGAGTAGAATCTAAAGGATTCACTAAGTGGACTCCAATGGAATAATCATCAAATGGAATC10.9900990099009901No Hit
ACACATCTCCGAGCCCACGAGACGTAGAGGATTCTCGTAAGGCGGCTTTTGCGTTAAAAAAAAAAACGAGGGCCG10.9900990099009901No Hit
AATTAACTCTTCTGAGAAGAAGTCTTTTTTATTCTGCCCATTATACATGCCATTCTCCTGGGCCTGTCTCTTATA10.9900990099009901No Hit
ATTATGGTTTAGTCTTAATAATAAAGATCCTTAAAAAATGAGATTAAGCGATTGAAAGAATCAAAACCACCCGTA10.9900990099009901No Hit
GCAATTCAGTCCAAAACATTTTACCTTCTGTCGTCCCAGAATTTATGCTCCTCTTGCATGCAAAATACATTCACC10.9900990099009901No Hit
TTACGGTAGCGCTCAATAAATTGGGGACTACATGCACTCTTGATGATGTTTTTCCTCCTGAGCATGATGGTCCAG10.9900990099009901No Hit
GTCCAGGACAACCACTCGCGCTCCATCAAGGGTGTGCTGCGTGGCCTGCACTACCAGGTGGAAAACACCCAAGGC10.9900990099009901No Hit
GTGCCCGAACTGCAAGGCCGCCTGCCGATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATG10.9900990099009901No Hit
TCTGTCGACGGTGGAGATTGCCGATTGGGTGCTCGTGCTGGAACCTGTCTCTTATACACATCTCCGAGCCCACGA10.9900990099009901No Hit
CAACACAAGATTAAATCAAGCAAAAGAGAAAATGTTGCAAACAAGAGATGCAATAAACATGAGCTGTATGAGGCT10.9900990099009901No Hit
AAAAACAACTGCACAAAGAAATAAATGAGGATAAAAAACTCTCGCTTAAACACATCACACCGCCCACTATAAAAC10.9900990099009901No Hit
GGACCTCCCGGCGACATGCGTAACCAACTTTACCGAAAATGGCCTTATGAACCCAACTCACCGCGCTGACAACCG10.9900990099009901No Hit
GCCAACCAGTTCGTCGCCAGCTTCATCGGCTCGCCGCCGATGAATTTCATCCCTGTCTCTTATACACATCTCCGC10.9900990099009901No Hit
AAATTATTTAATCTAGTTTGAGAAACAGTATATCGTATCATAAAAAACTACTTAAGGAATGCGAAGCATTGTGCA10.9900990099009901No Hit
CCCTAGAACTTAAAGTATAATGAAAAAGATCACTGATCACACATCACTATAATATATAATCTGTCTCTTATACAC10.9900990099009901No Hit
GACCCACATCGCCCCTGATCCGTTCGAGGAGTGTTTCGACGTTGACGAGGTAGTGCGACGGTTGTGCGCACGCAG10.9900990099009901No Hit
GATATAACGTAACTAAATTTTACAAATATTCTTTCACTTCCTGTGCTTTGGTCATACATGACGAAAAAAAAATAC10.9900990099009901No Hit
GCGCTCTCGCACGAAGGCCACGGGGCGCCGCTGCACCACGAACGGTTGTGGGACGACGAGTATGTATGCGTGATG10.9900990099009901No Hit
ATTATAAATCTATCTAATGACCACCGGTCGTCGGGAGACCCAGGAACACCTTGAAAGAATCATAGCTACAAGATT10.9900990099009901No Hit
GTCCCTACCCTCGCGAAAAAACAGTGCCGGCCGGAGCAGCAGCATGGGCAGTATCTACCTGATCAGACATGGTCA10.9900990099009901No Hit
ACTTGTAAGAGACTGCTGCAATGTTTTGATGAAGATATGCCCAAAGTAACATGAGAACCTGTCTCTTATACACAT10.9900990099009901No Hit
GTTCACCCGCCATCCGGGCGGCTTCGTGAACCGCGAGGGCGCAGTGCATCACGTCATCCAGGCGGCGATCGACCG10.9900990099009901No Hit
CCACCGTGCCTGGCTAATTTTTGTAATTTTTCTTGTACAGTTGGGGTTCTGTCTCTTATACACATCTCCGAGCCC10.9900990099009901No Hit
TCCAGGACTCTGGCACTTGGTGTTAGTTTGCTGTTGTTTCCACATAGGCAAACACTACCTCTGTCTCTTATACAC10.9900990099009901No Hit
ATGACGGGTATGCCTAACCGGTCGGCCAGGTCCATCACGCTCAGCGCATTCCCATAGCCCTCGGGCCGGGCGCTA10.9900990099009901No Hit
GGGCAATACAAGACTTACAGAAACTTGACAGTAGGTGGCCGATTGGGATACGTCTCCTCAAAGGTCAATACAAGT10.9900990099009901No Hit
TGATGCAGAAGCCGGACGTCGACCAGATCGACGGGCTCTCTCCGGCCATTTCCATCGATCAGAAAACGACTTCGA10.9900990099009901No Hit
GTTCGGACCTGGTAAGGGTTCCCGTGTTGGGGCAAATTAAGCCGCAGGCTCCACTCCTGGTGGTGCCCGTAAGTC10.9900990099009901No Hit
ACTTCGAAGACGCGAAGGACAAGATCTACATGGGTCCGGAGCGCAAGTCGGCCGTGATCCGCGAAGAGGAGCGCC10.9900990099009901No Hit
CTTATACCCATCTCCGAGCCCACGAGAACTCCCGTCCCCCAGTATGGATTCATCTGCTACGAAAACACGCGAGTA10.9900990099009901No Hit
GCAGAGATCCGAGATTGCGCCAATGCCCGCCATAATGGGTGACAGCGCAAGACTCCGTGTCGAAGAGAACTGAAA10.9900990099009901No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers