FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n01_zt14ss89.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n01_zt14ss89.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences92221
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGTTC150.002348664269.000013
TCGTATG600.063.25000440
TATGCCG950.061.73684343
CGCGTAT309.059913E-657.50000439
TAGAGCA252.5547823E-455.24
ACCGTTC252.5547823E-455.244
CTCGTAT700.054.21428739
GTATGCC1150.054.042
ATCTCGT850.052.76470637
ACACGAG200.007337701551.7553
CGTATGC1000.051.7541
TTGGACA200.007337701551.7511
GGGCTCT200.007337701551.7566
GACACGG200.007337701551.7545
AGGGTGC200.007337701551.756
ATTCCCG200.007337701551.7539
TGACCAT200.007337701551.7510
CGAGGAC200.007337701551.7532
GGTATGC200.007337701551.7541
AGGGCTA200.007337701551.7523
Produced by FastQC (version 0.11.2)