FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n01_zt14ss88.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n01_zt14ss88.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences133190
Sequences flagged as poor quality0
Sequence length75
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAACGA252.558925E-455.227
TCCGATA200.007344829451.758
TTCTTGT200.007344829451.7524
CATACCT200.007344829451.7515
ACAGATT200.007344829451.758
ATACAGT200.007344829451.756
GCGTATG704.3655746E-1044.35714340
CGTATGG651.1254087E-842.4615441
GGGTGTA1000.041.456
GGTGTAA1000.041.457
CCGATAA350.00134353439.4285749
GACTCGT350.00134353439.42857419
TCGCGTA801.6170816E-938.812538
AAGGGGG2450.036.61224769
AATCGGG3800.036.31579236
TGTGGGT1151.8189894E-1236.053
CGGGGTT1450.035.68965539
ATGAATC9800.035.20408233
GAATCTC1800.034.535
ACCTCCA400.002588133634.520
Produced by FastQC (version 0.11.2)