FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n01_zt14ss87.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n01_zt14ss87.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24349
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATGGC150.00232783169.042
TGGCGTG208.347916E-569.045
GAATCTC253.0042174E-669.035
CTGGTAT150.00232783169.039
TGTTGTG150.00232783169.050
CGTGTTG208.347916E-569.048
AGGGGGG150.00232783169.069
CTTTAGG150.00232783169.037
GGTTCCC150.00232783169.043
CGTTTGG150.00232783169.041
TGGTATG150.00232783169.040
AAGGGGG650.058.38461769
AATCTCG308.8613E-657.536
ATGGCGT252.5173542E-455.244
AATGGGG252.5173542E-455.269
GTGGAAA252.5173542E-455.258
GGGGTCT252.5173542E-455.249
GCGTGTT252.5173542E-455.247
CGCGTTT252.5173542E-455.239
GTGTTGT200.007273116651.7549
Produced by FastQC (version 0.11.2)