FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n01_zt14ss86.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n01_zt14ss86.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2451801
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACGCG8050.026.14280169
AATCGCG1004.3367952E-420.69995969
GGCGTAT850.00412154220.29407561
CGCCGTC1402.0838233E-417.24996656
ATGACGC1200.001477444217.24996467
ATCGCGA1200.001477444217.24996455
GGCGACG10050.017.16414565
GAATCGG2853.2923708E-1016.94767835
CGCTAAT4100.016.82923362
AGTCGCG1653.844522E-516.72723869
TCGCGAC1250.001941483616.55996556
GATCTTT32800.016.0929556
TAGACGT22300.015.9349444
CCGTCGA1756.3614236E-515.77139858
CTAGACG17350.015.7089013
CGAGTCG4400.015.68178767
CGATCTT58600.015.66037755
AATCGGG3101.0841177E-915.58093136
CGGGACG3101.0841177E-915.58061467
GGTCGCA2907.539711E-915.46548669
Produced by FastQC (version 0.11.2)