FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n01_zt14ss85.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n01_zt14ss85.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1363907
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTCGTA450.004622990730.66666614
CGATCTT25100.023.5039845
AATCGCG1052.281184E-522.99999836
CGCCAAC953.0660263E-421.78947434
AGTACCG800.002899707321.56255
ATCTGCG4450.020.9325857
GATCTTT22300.019.1838576
CGCATTC900.00573495519.16666612
TCTGCGC2801.2732926E-1118.4821438
TCGCACG1351.5772105E-417.8888910
ATCTCGC4950.017.4242427
CGTCCGC1200.001476968817.25000249
CGATCTG42500.017.1282375
CCGAAAA6050.017.10743917
ACTGATC7550.016.9072868
CCGATCT121200.016.7091584
TTCCGAT123050.016.3457952
CTGCGCT1907.0209135E-616.3421069
GATCTGG14450.016.2352946
ATCTTTT24750.016.0303047
Produced by FastQC (version 0.11.2)