FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n01_zt14ss84.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n01_zt14ss84.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1951518
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATCGT1455.296879E-721.4137926
GGTTCGT1004.336298E-420.78
TTCGGCT850.00412119720.29411741
TACGTCC850.00412119720.29411766
CGATCTT25500.020.1588235
GAATCGG1551.0052045E-620.03225969
TCCGGTA1258.7450084E-519.3219
CGATCTG35750.019.2041955
TCTGCGC2406.0936145E-1018.6875028
GTATAAG7050.018.1063821
ACCGTAG1150.001110600918.062
GTATTAA8650.017.5491311
ATCTGCG3750.017.487
TTCGTAG1200.001477277417.25000210
GTCTTAG5450.017.0917431
CTTAACG1250.00194126616.569
CCGATCT99850.016.3430164
GTATAGG4450.016.28091
GTATTAG7050.016.1489351
GTCCGGT1503.526705E-416.118
Produced by FastQC (version 0.11.2)