FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n01_zt14ss83.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n01_zt14ss83.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2116343
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTT30300.026.4157775
ACGAACC3750.021.1599559
CCGATCT130850.020.723684
TTCCGAT134150.020.2396092
TCCGATC135700.020.0592753
GCGCCAA4750.019.6104853
GATCTTT28200.019.574426
GCTACGC4800.019.40620213
GCTCCCG4650.019.29073337
CGATCTG50500.019.26535
CGATCTA17900.018.8882245
CTTCCGA144650.018.8658391
TCGGCTA4850.018.49480210
CGCCAAA4900.018.3060854
CGTTCAC4500.017.63329346
GGCTACG5100.017.58819412
ATCTTTT32850.017.0136597
CGGCTTA5500.016.93632342
CGGACGA4500.016.86662756
GGCGACT5350.016.76631512
Produced by FastQC (version 0.11.2)